Plaque assays were performed as previously described (43 (link)). Three days after infection with PR8-OVA lungs from infected mice were homogenized using a Fisher Tissuemiser, diluted in PBS and incubated on at least 80% confluent MDCK cells (ATCC) for 1 h at room temperature (RT). The wells were rinsed with PBS and a solution of 0.7% agarose, 0.1% trypsin in α-MEM was applied and allowed to solidify. Samples were then incubated at 37°C for 4 days before staining with crystal violet and counting of plaques.
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