In Vitro Drug Release of TAM-NE

The TAM-NE systems, which withstood the stability test and had a small droplet size < 100, namely NE1, NE9, and NE18, were further investigated for their in vitro drug release behaviours using the dialysis bag technique [8 (link),34 (link)]. Dialysis bags provided by Merck dialysis sacks (12–14 kDa) were filled with 1 mL of developed TAM-NE (NE1, NE9, and NE18) and subsequently suspended in a phosphate buffer medium of pH 7.4 (37 ± 0.5 °C). At a fixed time interval, 1 mL aliquots were taken and replaced by the same volume of fresh medium. The amount of TAM in the optimised formulations was quantified using UV-spectroscopy (λmax 235 nm). Experiments were carried out in triplicate.

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Alyami M.H., Alyami H.S., Alshehri A.A., Alsharif W.K., Shaikh I.A, & Algahtani T.S. (2022). Tamoxifen Citrate Containing Topical Nanoemulgel Prepared by Ultrasonication Technique: Formulation Design and In Vitro Evaluation. Gels, 8(7), 456.

Publication 2022

dialysis sacks

Buffer Dialysis Drug release Phosphate Spectroscopy

Corresponding Organization : Najran University

Other organizations : King Abdulaziz City for Science and Technology

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Variable analysis

independent variables

TAM-NE formulations (NE1, NE9, and NE18)

dependent variables

In vitro drug release behaviours
Amount of TAM in the optimised formulations

control variables

Dialysis bags (12–14 kDa) used to hold the TAM-NE formulations
Phosphate buffer medium of pH 7.4 (37 ± 0.5 °C) used as the release medium
Aliquot size of 1 mL taken at fixed time intervals and replaced with the same volume of fresh medium

controls

No positive or negative controls were explicitly mentioned.

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