Protocol detail

Isolation and Cultivation of Babesia divergens

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Strains of B. divergens were isolated from bovine blood during the acute phases of babesioses as described earlier [25 (link)]. 11 isolates of B. divergens from different geographical locations within France were cultivated and cloned by limited dilution [26 (link)]. The first two digits in the description of each clone (Additional file 1: Table S1) refer to the French county of origin. Isolate Rouen 87 originated from human blood [27 (link)]. Babesia divergens isolates were cultivated in vitro in a suspension of bovine erythrocytes obtained from a parasite-free cow (serologically negative and culture tested) as described [25 (link), 26 (link)]. Parasitemia was monitored using the commercial Diff-Quik Stain Set (Siemens) and RBC smears.

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Jalovecka M., Bonsergent C., Hajdusek O., Kopacek P, & Malandrin L. (2016). Stimulation and quantification of Babesia divergens gametocytogenesis. Parasites & Vectors, 9, 439.

Publication 2016

Diff-Quik Stain Set

Babesia Babesioses Blood Clone Diff quik stain Dilution First digits Human Parasite Parasitemia Strains

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Variable analysis

independent variables

Strains of B. divergens isolated from bovine blood during the acute phases of babesioses

dependent variables

Parasitemia monitored using the commercial Diff-Quik Stain Set (Siemens) and RBC smears

control variables

Bovine erythrocytes obtained from a parasite-free cow (serologically negative and culture tested)

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