Pregnancy Effects on Human Islet Grafts

Upon receipt, human islets and human ductal aggregates isolated from nondiabetic donors (n = 4) were cultured overnight in Miami Media 1A (Cellgro). Hand-picked and size-matched islets (1000 IEQ) were transplanted together with 100 human ductal aggregates (cluster of ductal cells) under the kidney capsule of both NSG-Lox and NSG-LIRKO mice as described previously (56 (link), 57 (link)). After allowing 10 days after transplantation for islet engraftment, mice were either maintained in a nonpregnant state or allowed to breed to become pregnant. Human islet and duct grafts were removed on pregnancy day 15.5 and snap-frozen for further analysis.

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Dirice E., Basile G., Kahraman S., Diegisser D., Hu J, & Kulkarni R.N. (2022). Single-nucleus RNA-Seq reveals singular gene signatures of human ductal cells during adaptation to insulin resistance. JCI Insight, 7(16), e153877.

Publication 2022

Miami Media 1A

Capsule Cells Donors Frozen Grafts Human Kidney Mice Pregnancy Transplantation

Corresponding Organization : Harvard Stem Cell Institute

Other organizations : New York Medical College

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Variable analysis

independent variables

Pregnancy state (non-pregnant or pregnant)

dependent variables

Human islet and duct graft survival/function

control variables

Donor age (n = 4)
Transplantation site (under the kidney capsule)
Mouse strain (NSG-Lox and NSG-LIRKO)
Transplantation duration (10 days)

positive controls

Not specified

negative controls

Not specified

Annotations

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