Diverse Chemical Compounds Protocol

Dactinomycin and piplartine were purchased from Selleck Chemicals (Houston TX); Melatonin^{22 (link)}, Trichostatin A^{26 (link)}, γ-Tocotrienol^{25 (link)}, Auranofin^{18 (link)}, Genistein^{27 (link)}, and Mito-Tempo^{20 (link)} from Cayman chemicals (Ann Arbor, MI); GC1149^{19 (link)} from Galera Therapeutics (Malvern, PA); Recilisib sodium (also named as Ex-Rad)^{17 (link)} from MedKoo Biosciences (Morrisville, NC; V028–5832 (also named as compound C)^{21 (link)} and Y600–0815(also named as PKUMDL-LC-101)^{23 (link)} from Enamine, (Monmouth Junction, NJ). The stock solutions of all these chemicals at 10 mM in DMSO were prepared, unless specified otherwise.
Cell-permeable Hoechst 33342 dye, and Calcein-AM were from Thermo Fisher Scientific (Waltham, MA). 1 mM and 2 mM (1000x) stock solutions were prepared according to the vender’s instructions, respectively.

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Wen K.K., Roy S., Grumbach I.M, & Wu M. (2021). A “failed” assay development for the discovery of rescuing small molecules from the radiation damage. SLAS discovery : advancing life sciences R & D, 26(10), 1315-1325.

Publication 2021

Dactinomycin Melatonin Trichostatin A γ-Tocotrienol Auranofin Genistein Mito-Tempo Calcein-AM

Calcein Cayman Cell Dactinomycin Dmso Ex rad Hoechst 33342 Mito Permeable Piplartine Sodium Therapeutics Trichostatin

Corresponding Organization : University of Iowa

Top 5 similar protocols

Variable analysis

independent variables

Dactinomycin
Piplartine
Melatonin
Trichostatin A
γ-Tocotrienol
Auranofin
Genistein
Mito-Tempo
GC1149
Recilisib sodium (Ex-Rad)
V028–5832 (compound C)
Y600–0815 (PKUMDL-LC-101)

dependent variables

Cell viability
Cell survival

control variables

Cell-permeable Hoechst 33342 dye
Calcein-AM

Annotations

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