Culturing Diverse Microbial and Mammalian Cells

M. luteus (ATCC 4698) and S. aureus (ATCC 25923) were cultured in tryptic soy broth. GFP-E. coli was generated as described^{32 (link)} and cultured in LB medium. Drosophila S2 cells (RRID: CVCL_TZ72) and S2R+ cells (RRID: CVCL_Z831) were cultured at 25 °C in Drosophila Schneider’s Medium (Biological Industries) supplemented with 10% FBS and antibiotics. Human HEK293T cells (ATCC#CRL-11268) and mouse MEF cells (ATCC#CRL-2991) were cultured at 37 °C in DMEM medium (Sigma) supplemented with 10% FBS and antibiotics. Human THP-1 cells (ATCC#TIB-202) were cultured at 37 °C in RPMI 1640 medium (Sigma) supplemented with 10% FBS and antibiotics.

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Yang Y., Zhou H., Huang X., Wu C., Zheng K., Deng J., Zheng Y., Wang J., Chi X., Ma X., Pan H., Shen R., Pan D, & Liu B. (2024). Innate immune and proinflammatory signals activate the Hippo pathway via a Tak1-STRIPAK-Tao axis. Nature Communications, 15, 145.

Publication 2024

Human HEK293T cells DMEM medium Human THP-1 cells RPMI 1640 medium

Corresponding Organization :

Other organizations : Xiamen University, The University of Texas Southwestern Medical Center, Howard Hughes Medical Institute, Westlake University

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Variable analysis

independent variables

M. luteus (ATCC 4698)
S. aureus (ATCC 25923)
GFP-E. coli
Drosophila S2 cells (RRID: CVCL_TZ72)
Drosophila S2R+ cells (RRID: CVCL_Z831)
Human HEK293T cells (ATCC#CRL-11268)
Mouse MEF cells (ATCC#CRL-2991)
Human THP-1 cells (ATCC#TIB-202)

dependent variables

Unclear from the information provided

control variables

Tryptic soy broth
LB medium
Drosophila Schneider's Medium (Biological Industries) supplemented with 10% FBS and antibiotics
DMEM medium (Sigma) supplemented with 10% FBS and antibiotics
RPMI 1640 medium (Sigma) supplemented with 10% FBS and antibiotics
Temperature: 25 °C for Drosophila cells, 37 °C for human and mouse cells

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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