Neuroinflammation Modulation Reagent Study

THP (purity ≥99.0%) was purchased from Shanghai Selleck Chemicals (Shanghai, China). CFA and lipopolysaccharides were purchased from Sigma (St. Louis, United States). Primary antibodies against GFAP, Iba-1, NF-κB, p-NF-κB and GAPDH were purchased from Abcam (Cambridge, UK). All secondary antibodies conjugated with horseradish peroxidase (HRP) were purchased from Beyotime (Shanghai, China). Fetal bovine serum was purchased from Gibco (St. Louis, United States). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Corning (Manassas, USA). All of the chemicals and reagents used were of standard biochemical quality.^{12 (link)}

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Liu B., Luo M., Meng D., Pan H., Shen H., Shen J., Yao M, & Xu L. (2021). Tetrahydropalmatine exerts analgesic effects by promoting apoptosis and inhibiting the activation of glial cells in rats with inflammatory pain. Molecular Pain, 17, 17448069211042117.

Publication 2021

CFA lipopolysaccharides Primary antibodies against GFAP p-NF-κB GAPDH Fetal bovine serum Dulbecco’s modified Eagle’s medium (DMEM)

Antibodies Eagle Fetal bovine serum Gapdh Gfap Horseradish peroxidase Lipopolysaccharides Nf κb

Corresponding Organization : Jiaxing University

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Variable analysis

independent variables

THP (purity ≥99.0%)
Lipopolysaccharides

dependent variables

Iba-1
NF-κB
P-NF-κB
GAPDH

control variables

Fetal bovine serum
Dulbecco's modified Eagle's medium (DMEM)
All of the chemicals and reagents used were of standard biochemical quality

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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