Myelin content and integrity were assessed using Luxol fast blue (LFB) staining, following previously described methods.
36 (link) Tissue slides were incubated with LFB from Sigma in acidified 95% alcohol at 60°C overnight. After being washed with 95% alcohol and ddH2O, the sections underwent immersion in a lithium carbonate solution, followed by exposure to a cresyl violet solution for differentiation and counterstaining. The images of LFB staining were acquired using a white light microscope, and the optical density was calculated by ImageJ.
36 (link) Tissue slides were incubated with LFB from Sigma in acidified 95% alcohol at 60°C overnight. After being washed with 95% alcohol and ddH2O, the sections underwent immersion in a lithium carbonate solution, followed by exposure to a cresyl violet solution for differentiation and counterstaining. The images of LFB staining were acquired using a white light microscope, and the optical density was calculated by ImageJ.
Free full text: Click here
