Automated High-Throughput DNA Extraction from Mouse Stool

DNA isolation was performed as previously described (23 (link)–25 (link)). Mouse stool samples were transferred to a 2 ml tube containing 200 mg of ≤106 μm glass beads (Sigma, St. Louis, MO) and 0.5 ml of Qiagen PM1 buffer (Valencia, CA). Bead beating was performed for 5 min in a Qiagen TissueLyser II at 30Hz. After a 5-min centrifugation, 0.45 ml of supernatants were aspirated and transferred to a new tube containing 0.15 ml of Qiagen IRS solution. The suspension was incubated at 4°C overnight. After a brief centrifugation, supernatants were aspirated and transferred to deep well plates containing 0.45 ml of Qiagen binding buffer supplemented with Qiagen ClearMag Beads. DNA was purified using the automated KingFisher™ Flex Purification System and eluted in DNase-free water.