Urine biomarker levels were measured at the Cincinnati Children's Hospital Medical Center Biomarker Laboratory. Commercially available ELISA kits were used to measure urine IL-18 (Medical & Biological Laboratories Co., Nagoya, Japan), KIM-1 (R & D Systems, Inc., Minneapolis, MN),26 (link) and PIIINP (USCN Life Sciences, Wuhan, Hubei, China). Urine albumin and creatinine were measured by immunoturbidimetry and colorimetric enzyme assay, respectively, using a Siemens Dimension Xpand plus HM clinical analyzer (Siemens, Munich, Germany).
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Urine Biomarker Measurement Protocol
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Albumin
Biological
Biomarker
Colorimetric
Creatinine
Elisa
Enzyme assay
Il 18
Immunoturbidimetry
Kim 1
Piiinp
Urine
Corresponding Organization : University of California, San Francisco
Other organizations : Johns Hopkins University, The Lundquist Institute, Harbor–UCLA Medical Center, Northwestern University, University of Pittsburgh, Cincinnati Children's Hospital Medical Center, Yale University, University of California, San Diego, VA San Diego Healthcare System
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Variable analysis
independent variables
- Not explicitly mentioned
- Urine IL-18
- Urine KIM-1
- Urine PIIINP
- Urine albumin
- Urine creatinine
- Not explicitly mentioned
- No positive or negative controls were specified by the authors.
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