Cells were seeded at a density to reach 30–50% confluence by the day of transfection. Cells were transfected as described previously [45 (link)] with small interfering RNA (siRNA) against HIF-1α (siHIF-1α) (Ambion/Life Technologies) using Oligofectamine (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Non-targeting siRNA, were used as a negative control. Optimal knockdown was verified by western blot.
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