Live Cell Imaging of Mitotic Progression

Live cell imaging for Fig. 2E was performed with a DeltaVision microscope as previously described (Vagnarelli et al., 2011 (link)).
For Fig. 4G,H and Fig. S2, HeLa cells were seeded onto Labtech chamber slides with complete Leibovitz's L-15 medium, lacking phenol red (Gibco). Cells were imaged by differential interference contrast microscopy with a 20× objective (NA 0.45) and a wide-field microscope (NIKON Ti-E super research Live Cell imaging system) at 37°C. Images were captured every 30 min over a period of 24.5 h. Analyses of mitotic progression were conducted manually.

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Stamatiou K., Chmielewska A., Ohta S., Earnshaw W.C, & Vagnarelli P. (2023). CCDC86 is a novel Ki-67-interacting protein important for cell division. Journal of Cell Science, 136(2), jcs260391.

Publication 2023

phenol red Ti-E super research Live Cell imaging system

Cell Differential interference contrast microscopy Hela cells Microscope Progression

Corresponding Organization :

Other organizations : Brunel University of London, Wellcome Centre for Cell Biology, University of Edinburgh, Hokkaido University

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Variable analysis

independent variables

Live cell imaging technique used (DeltaVision microscope, differential interference contrast microscopy with a 20× objective, wide-field microscope)

dependent variables

Mitotic progression

control variables

Incubation temperature (37°C)
Culture medium (complete Leibovitz's L-15 medium, lacking phenol red)
Cell line (HeLa cells)
Imaging time interval (30 min over a period of 24.5 h)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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