Rapid RNA Stabilization Filtration Tests

We performed preliminary filtration tests using water samples from the North Western Mediterranean Sea (41 39.7 N 02 54.6 E). Based on previous estimation of Prochlorococcus mRNA half-live rates [67 (link)], we set a maximum operation time of 10 minutes from sampling to RNA stabilization, and all parameters were adjusted to this limit. We tested three different 47-mm-diameter, 0.2-μm-pore-size filters: Nucleopore polycarbonate (Whatman, Freiburg, GE), Durapore PVDF (Millipore, Billerica, MA) and Omnipore PTFE (Millipore). Finally, for each filter type we evaluated the saturation capacity, cell retention and ease of use for nucleic acids isolation.

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Fernández-Pinos M.C., Casado M., Caballero G., Zinser E.R., Dachs J, & Piña B. (2015). Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. PLoS ONE, 10(8), e0133207.

Publication 2015

Nucleopore polycarbonate Durapore PVDF

Cell Filtration Isolation Mrna Nucleic acids Nucleopore Polycarbonate Prochlorococcus Ptfe Pvdf Retention

Corresponding Organization :

Other organizations : Consejo Superior de Investigaciones Científicas, University of Tennessee at Knoxville

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Variable analysis

independent variables

Three different 47-mm-diameter, 0.2-μm-pore-size filters: Nucleopore polycarbonate (Whatman, Freiburg, GE), Durapore PVDF (Millipore, Billerica, MA) and Omnipore PTFE (Millipore)

dependent variables

Saturation capacity of each filter type
Cell retention of each filter type
Ease of use for nucleic acids isolation for each filter type

control variables

Maximum operation time of 10 minutes from sampling to RNA stabilization

positive controls

Not mentioned

negative controls

Not mentioned

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