Isolation and Characterization of Exosomes from HCC Tissues

Cells were isolated from cancer and para-carcinoma tissues of HCC patients as previously described (18 (link)). Transmission electron microscopy (TEM) was used to identify exosomes structures. Exosomes size was calculated using Zetasizer Nano ZS. Exosomes were analyzed using exosome marker protein CD63, Alix, and Tsg101 via Western blot. CD8+ T cells purified from serum of healthy donor or HCC patients using Easy-SepTM Direct Human CD8+ T-Cell Isolation Kit (STEMCELL Technologies) as previous described (19 (link)).

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Zhang H.Y., Liang H.X., Wu S.H., Jiang H.Q., Wang Q, & Yu Z.J. (2021). Overexpressed Tumor Suppressor Exosomal miR-15a-5p in Cancer Cells Inhibits PD1 Expression in CD8+T Cells and Suppresses the Hepatocellular Carcinoma Progression. Frontiers in Oncology, 11, 622263.

Publication 2021

Easy-SepTM Direct Human CD8+ T-Cell Isolation Kit

Cancer Carcinoma Cd8 t cell Cells Donor Exosome Human Isolation Patients Protein Serum Stemcell Tissues Transmission electron microscopy Tsg101 Western blot

Corresponding Organization : First Affiliated Hospital of Zhengzhou University

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Variable analysis

independent variables

Cancer and para-carcinoma tissues of HCC patients

dependent variables

Exosome structures identified using transmission electron microscopy (TEM)
Exosome size calculated using Zetasizer Nano ZS
Exosome marker proteins CD63, Alix, and Tsg101 analyzed via Western blot

control variables

CD8+ T cells purified from serum of healthy donors
CD8+ T cells purified from serum of HCC patients

controls

Positive control: CD8+ T cells purified from serum of healthy donors
Negative control: CD8+ T cells purified from serum of HCC patients

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