Adrenal Cortical Cell Culture Infection
Corresponding Organization : University of Colorado Denver
Variable analysis
- Presence or absence of VZV infection (mock or VZV-infected qHAdCCs)
- Cell harvesting at 1, 2, or 3 days post-infection (DPI)
- Conditioned supernatant collection at 3 DPI
- Cell type (primary human adrenal cortical cells, HAdCCs)
- Cell seeding density (5000 cells/cm^2)
- Culture media composition (mesenchymal medium with 5% FBS, 1% mesenchymal stem cell growth supplement, and 1% penicillin-streptomycin)
- Media change to 0.1% FBS and 1% penicillin-streptomycin after 24 h
- Media replenishment every 72 h for 7 days to establish quiescence (qHAdCC)
- VZV strain (Gilden strain [GenBank accession number MH379685])
- VZV infection dose (60 plaque-forming units/cm^2)
- Uninfected (mock) qHAdCCs
Annotations
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