Sequencing and Annotation of DisaGV Genome

DisaGV genomic DNA was sequenced with the 454 Genome Sequencer (GS) FLX™ Standard (Roche) at the Centro de Genômica de Alto Desempenho do Distrito Federal (Brasília, Brazil). The genome was assembled de novo using Geneious 7.0 [47 (link)] and confirmed by comparing the restriction endonuclease fragment pattern from digestion of viral DNA to the fragment pattern generated from the assembled sequence. The annotation was performed using Geneious 7.0 to identify the ORFs that started with a methionine codon (ATG) encoding at least 50 amino acids and blastp to identify homologs. Specific primers were designed to amplify and sequence, by Sanger method, all regions in the genome with low coverage (<10 x).