Protocol detail

Culturing Murine and Human Cell Lines

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Murine astrocytoma ALTS1C1 cells (BCRC60582, Taiwan) [22 (link)], murine pancreatic UN-KC6141 cells (kindly provided by Prof. Batra, S. K. from Department of Pathology and Microbiology, University of Nebraska Medical Center, USA) [23 (link)], and human glioma U87-MG cells (ATCC No. CRL-9589, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO, Thermo Fisher Scientific, Inc., USA) with 10% fetal bovine serum (GIBCO), and 1% penicillin–streptomycin (GIBCO) at 37 °C in a humidified 5% CO₂/air atmosphere.

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Liu C.C., Yu C.F., Wang S.C., Li H.Y., Lin C.M., Wang H.H., Abate C, & Chiang C.S. (2019). Sigma-2 receptor/TMEM97 agonist PB221 as an alternative drug for brain tumor. BMC Cancer, 19, 473.

Publication 2019

U87-MG cells Dulbecco’s modified Eagle’s medium fetal bovine serum penicillin–streptomycin

Astrocytoma Atmosphere Cells Eagle Fetal bovine serum Glioma Human Murine Pancreatic Penicillin Streptomycin

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Variable analysis

independent variables

Cell lines used: Murine astrocytoma ALTS1C1 cells, murine pancreatic UN-KC6141 cells, and human glioma U87-MG cells

dependent variables

Not explicitly mentioned

control variables

Dulbecco's modified Eagle's medium (GIBCO, Thermo Fisher Scientific, Inc., USA) with 10% fetal bovine serum (GIBCO), and 1% penicillin–streptomycin (GIBCO)
Incubation at 37 °C in a humidified 5% CO2/air atmosphere

controls

No positive or negative controls were explicitly mentioned in the provided information.

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