Caco-2 Cells with Cetuximab Resistance

Caco-2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 1% Pen/Strep. For the establishment of CTX resistant Caco-2 clones (Caco-2 CXR) please refer to our previous work [21 (link)]. Caco-2 CXR were maintained in presence of cetuximab (Erbitux, Merck KgaA, Germany) at 10 µg/mL concentration. Caco-2 cells were authenticated by short tandem repeat (STR) profiling (PowerPlex 21 PCR Kit, Promega, Madison, WI, USA) and the certificate was released by the external service Eurofins Medigenomix Forensik GmbH (Ebersberg, Germany). Cells were routinely tested for mycoplasma contamination.

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Gelfo V., Pontis F., Mazzeschi M., Sgarzi M., Mazzarini M., Solmi R., D’Uva G, & Lauriola M. (2019). Glucocorticoid Receptor Modulates EGFR Feedback upon Acquisition of Resistance to Monoclonal Antibodies. Journal of Clinical Medicine, 8(5), 600.

Publication 2019

Erbitux PowerPlex 21 PCR Kit

Caco 2 cells Cells Cetuximab Clones Erbitux Fetal bovine serum Mycoplasma Promega Short tandem repeat Strep

Corresponding Organization : Policlinico S.Orsola-Malpighi

Other organizations : Istituti di Ricovero e Cura a Carattere Scientifico, MultiMedica

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Variable analysis

independent variables

Cetuximab (Erbitux, Merck KgaA, Germany) at 10 µg/mL concentration

dependent variables

Establishment of CTX resistant Caco-2 clones (Caco-2 CXR)

control variables

DMEM supplemented with 10% fetal bovine serum and 1% Pen/Strep for maintaining Caco-2 cells
Caco-2 cells authenticated by short tandem repeat (STR) profiling
Cells routinely tested for mycoplasma contamination

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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