Isolation and Characterization of Glucosinolates

D,L-SFN (1-isothiocyanato-4-(methylsulfinyl)-butane) and the potassium salt of its GLS precursor GRN (4-methylsulfinylbutylglucosinolate), DEAE-Sephadex A-25 and Sephadex LH-20, formic acid, ammonia, acetonitrile, sinigrin, L-ascorbic acid, MES, BSA, Mueller Hinton Broth No. 2, 9-aminoacridine, AMP, ATP, glucose-6-phosphate, acetyl-CoA, TFA were all from Sigma-Aldrich and supplied via MERCK spol. S.r.o., (Bratislava, Slovakia); peptone for bacteriology, agar (Biolife Italiana, Milano, Italy). Unless otherwise noted in the text, all chemicals were from MERCK and were of analytical grade.

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Polozsányi Z., Galádová H., Kaliňák M., Jopčík M., Kaliňáková B., Breier A, & Šimkovič M. (2024). The Antimicrobial Effects of Myrosinase Hydrolysis Products Derived from Glucosinolates Isolated from Lepidium draba. Plants, 13(7), 995.

Publication 2024

DEAE-Sephadex A-25 Sephadex LH-20 formic acid acetonitrile sinigrin L-ascorbic acid BSA 9-aminoacridine ATP glucose-6-phosphate acetyl-CoA

Corresponding Organization : Slovak University of Technology in Bratislava

Other organizations : Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences, Slovak Academy of Sciences, Centre of Biosciences of the Slovak Academy of Sciences

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Variable analysis

independent variables

D,L-SFN (1-isothiocyanato-4-(methylsulfinyl)-butane)
Potassium salt of its GLS precursor GRN (4-methylsulfinylbutylglucosinolate)
DEAE-Sephadex A-25
Sephadex LH-20
Formic acid
Ammonia
Acetonitrile
Sinigrin
L-ascorbic acid
Mueller Hinton Broth No. 2
9-aminoacridine
Glucose-6-phosphate
Acetyl-CoA

dependent variables

Not explicitly mentioned

control variables

Unless otherwise noted in the text, all chemicals were from MERCK and were of analytical grade
Peptone for bacteriology
Agar (Biolife Italiana, Milano, Italy)

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