Combination Therapy for Colorectal Cancer

Female NMRI nu/nu mice (Charles River, 12 weeks old) received s.c. injections of 3×10⁶ Colo205 cells in 100 µl PBS at the left and right dorsal sides. Treatment started 14 days after tumour cell inoculation when tumours reached an average volume of 100 mm³. 0.3 nmol HC-scTRAIL or Fc-scTRAIL-FAVSGAA were administered twice a week in 100 µl PBS for a total of six doses. The control group received six doses of 100 µl PBS. In a second round of treatment, 100 µg Smac mimetic SM83 was co-administered i.p., together with a total of 4 i.v. doses of PBS or 0.3 nmol of the proteins twice a week. Tumour volume was monitored as described^{9 (link)}. One-way ANOVA and Tukey post hoc tests were performed for statistical analysis.