As an example of real RNA-seq data with known expression profiles, data generated by the SEQC Project (2 (link)) was used. Two particular FASTQ files were used, one generated from sequencing of Human Brain Reference RNA (HBRR) and one from Universal Human Reference RNA (UHRR). Each file contains 15 million 100 bp read-pairs and was generated from an Illumina HiSeq sequencer.
The SEQC Project includes expression values measured by TaqMan RT-PCR for slightly over 1000 genes for both HBRR and UHRR. 958 of these TaqMan validated genes were found to have matched symbols with genes in the RNA-seq data. The TaqMan RT-PCR expression values are available from the seqc Bioconductor package.
The SEQC Project includes expression values measured by TaqMan RT-PCR for slightly over 1000 genes for both HBRR and UHRR. 958 of these TaqMan validated genes were found to have matched symbols with genes in the RNA-seq data. The TaqMan RT-PCR expression values are available from the seqc Bioconductor package.
