For the p-tau212 assay, which was developed on the Simoa HD-X instrument (Quanterix, MA, USA), the p-tau212 antibody was used as the capture antibody. A mouse monoclonal antibody raised against the N-terminal region of tau (Tau12; BioLegend, #SIG-39416) was used for detection. In vitro phosphorylated recombinant full-length tau-441 (#269022, Abcam) was used as the assay calibrator. Blood samples and calibrators were diluted with assay diluent (Tau 2.0 Sample Diluent; #101556, Quanterix).
Analytical validation of the p-tau212 assay followed protocols described previously8 (link),10 (link),31 ,39 (link). Assay development work was conducted at the University of Gothenburg, Sweden. In the clinical studies, p-tau212 was measured at the University of Gothenburg by scientists blinded to participant information, using the above-described assays. For p-tau212, plasma and CSF samples were diluted 1.2 times and 10 times respectively prior to measurement.
Analytical validation of the p-tau212 assay followed protocols described previously8 (link),10 (link),31 ,39 (link). Assay development work was conducted at the University of Gothenburg, Sweden. In the clinical studies, p-tau212 was measured at the University of Gothenburg by scientists blinded to participant information, using the above-described assays. For p-tau212, plasma and CSF samples were diluted 1.2 times and 10 times respectively prior to measurement.
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