Quantitative PCR for M. leprae
Corresponding Organization : Universidade Federal de Uberlândia
Variable analysis
- DNA extraction from dermal smear samples and biopsies (nerve and overlying skin)
- Detection of M. leprae DNA using quantitative real-time polymerase chain reaction (qPCR) primer/probe assay targeting the M. leprae species-specific genomic region (RLEP3)
- Reactions performed on the ABI7300 platform (Applied Biosystems)
- Results analyzed using the 7,300 System SDS Software vs. 1.4
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!