After 4 weeks of electroacupuncture treatment in each group of mice, inhaled isoflurane anesthetic fully exposed the liver of the mice, and the left leaf of the liver was placed in liquid nitrogen for follow-up testing. After liver sample collection, mice were euthanized by cervical dislocation. Each mouse took 50 mg of liver tissue and added liver tissue and lye to the test tube at 1 : 3 as hydrolysate, boiling water bath for 20 min, flowing water cooling. The liver glycogen hydrolysate was configured into 1% liver glycogen assay solution, and the assay reagents were configured according to Table 2, mixed and boiled in a water bath for 5 min, cooled, and colorimetric at 620 nm wavelength; the OD value of each tube was measured; the standard curve was drawn; the concentration of liver tissue homogenate was calculated from the standard curve; and the liver glycogen content was calculated by the formula.
The formula for calculating liver glycogen content is as follows: Liver glycogen contentmggtissue=Measure the OD value of the tubeStandard TUBE OD value×standard tube content0.01mg×dilution ratio of the sample before test×10÷1.11.
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