Fish Anesthesia and Morphological Analysis

Before examination, the fish was anesthesia using 0.0125% buffered MS-222 solution (ethyl 3-aminobenzoate methane sulfonic acid salt, Sigma-Aldrich, St. Louis, MI, USA). Total length and standard length were measured using Vernier calipers with 0.1 mm accuracy. Fish body weight was determined to an accuracy of 0.01 g. For morphological analysis, a digital CMOS X-ray detector (Model 2315, Dexela, London, UK), set to 45 kV and 120 mA, with 2.5 seconds of exposure, was used to view vertebrae of the fish [19 (link)].

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Hsu P.J., Wang H.D., Tseng Y.C., Pan S.W., Sampurna B.P., Jong Y.J, & Yuh C.H. (2021). l-Carnitine ameliorates congenital myopathy in a tropomyosin 3 de novo mutation transgenic zebrafish. Journal of Biomedical Science, 28, 8.

Publication 2021

MS-222 solution

Anesthesia Body weight Cmos Digital x ray Ethyl 3 aminobenzoate Fish Methane sulfonic acid Ms 222 Salt Seconds Vertebrae

Corresponding Organization :

Other organizations : National Health Research Institutes, National Tsing Hua University, Institute of Cellular and Organismic Biology, Academia Sinica, National Yang Ming Chiao Tung University

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Variable analysis

independent variables

Anesthesia using 0.0125% buffered MS-222 solution

dependent variables

Total length
Standard length
Fish body weight

control variables

Vernier calipers with 0.1 mm accuracy
Digital CMOS X-ray detector set to 45 kV and 120 mA, with 2.5 seconds of exposure

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