L929 fibroblasts (5 × 105 cells/well) were cultured in DMEM (Dulbecco’s Modified Eagle Medium, Sigma-Aldrich) [120 (link)] medium supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 1% pen/strep (penicillin/streptomycin solution, Sigma Aldrich) for 24 h at 37 °C in 95% humidity with 5% CO2.
The samples were co-cultured with the fibroblasts for 24 h (37 °C, 95% humidity, 5% CO2). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) [121 ] assay was used to evaluate the cell viability and proliferation in the presence of the materials. Cells were incubated for 4 h with MTT reagent (Vybrant® MTT Cell Proliferation Assay Kit, V-13154) at 37 °C in 95% humidity with 5% CO2. After incubation, formazan crystals were solubilized with DMSO for 10 min at room temperature. The absorbance was measured at λ = 540 nm using the Multiskan FC apparatus (Life Technologies Holdings Pte. Ltd., a part of Thermo Fisher Scientific Inc., Singapore). The cell morphology was evaluated using an inverted Olympus IX73 microscope after 24 h of incubation with the biomaterials.
The samples were co-cultured with the fibroblasts for 24 h (37 °C, 95% humidity, 5% CO2). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) [121 ] assay was used to evaluate the cell viability and proliferation in the presence of the materials. Cells were incubated for 4 h with MTT reagent (Vybrant® MTT Cell Proliferation Assay Kit, V-13154) at 37 °C in 95% humidity with 5% CO2. After incubation, formazan crystals were solubilized with DMSO for 10 min at room temperature. The absorbance was measured at λ = 540 nm using the Multiskan FC apparatus (Life Technologies Holdings Pte. Ltd., a part of Thermo Fisher Scientific Inc., Singapore). The cell morphology was evaluated using an inverted Olympus IX73 microscope after 24 h of incubation with the biomaterials.
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