Expression
of WT and mutant HA-H4R
on the surface of intact HEK293 cells was detected by ELISA using
1:800 anti-HA high-affinity clone 3F10 primary antibody (Sigma-Aldrich)
and 1:5000 horseradish peroxidase-conjugated goat anti-rat secondary
antibody (Thermo Fisher Scientific), as previously described.67 (link) Peroxidase activity was measured using 3,3′5,5′-tetramethylbenzidine
liquid substrate system (Abcam; Cambridge, UK) on a Victor3 1420 multilabel
plate reader (PerkinElmer) at 450 nm.
of WT and mutant HA-H4R
on the surface of intact HEK293 cells was detected by ELISA using
1:800 anti-HA high-affinity clone 3F10 primary antibody (Sigma-Aldrich)
and 1:5000 horseradish peroxidase-conjugated goat anti-rat secondary
antibody (Thermo Fisher Scientific), as previously described.67 (link) Peroxidase activity was measured using 3,3′5,5′-tetramethylbenzidine
liquid substrate system (Abcam; Cambridge, UK) on a Victor3 1420 multilabel
plate reader (PerkinElmer) at 450 nm.
