Transcription Analysis of NK Receptors

Expression analysis was performed as previously described.⁵⁰ (link) NKp30, NKp44, NKp46, DNAM-1, and CD161 transcripts were determined with specific primers in end-point PCR using 5´FAM-labeled reverse primers (primers and protocols can be obtained on request). PCR products were analyzed for fragment length in an ABI sequencer with Gene Scan-600 LIZ for length standard and GeneMapper software (both Applied Biosystems, Germany); mean fluorescence intensity (MFI) was used for semiquantitative analysis.

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Schilbach K., Alkhaled M., Welker C., Eckert F., Blank G., Ziegler H., Sterk M., Müller F., Sonntag K., Wieder T., Braumüller H., Schmitt J., Eyrich M., Schleicher S., Seitz C., Erbacher A., Pichler B.J., Müller H., Tighe R., Lim A., Gillies S.D., Strittmatter W., Röcken M, & Handgretinger R. (2015). Cancer-targeted IL-12 controls human rhabdomyosarcoma by senescence induction and myogenic differentiation. Oncoimmunology, 4(7), e1014760.

Publication 2015

Gene Scan-600 LIZ GeneMapper software

Cd161 Dnam 1 Fluorescence Gene Nkp44 Nkp46 Primers Scan

Corresponding Organization : Children's Clinical University Hospital

Other organizations : Imaging Center, Siemens (Germany), Stem Cell Institute, University of Würzburg, Taipei Institute of Pathology, Merck (Germany), Institut Pasteur, Merck Serono (Switzerland), Center for Global Development

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Variable analysis

independent variables

NKp30
NKp44
NKp46
DNAM-1
CD161

dependent variables

Transcript levels of NKp30, NKp44, NKp46, DNAM-1, and CD161

control variables

Primers and protocols used for end-point PCR

controls

No positive or negative controls were explicitly mentioned.

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