Immunocytochemistry was performed as described previously (17 (link)). Omission of the primary antibodies and replacement with mouse, rabbit and sheep isotype IgGs were used as negative control. Primary antibodies and secondary antibodies are described in
Meiotic Spread Preparation and Immunocytochemistry
Immunocytochemistry was performed as described previously (17 (link)). Omission of the primary antibodies and replacement with mouse, rabbit and sheep isotype IgGs were used as negative control. Primary antibodies and secondary antibodies are described in
Corresponding Organization : University of Amsterdam
Variable analysis
- Preparation method for meiotic spread preparations (either as previously described or using Cytospin)
- Not explicitly mentioned
- Spermatocytes (in vivo or in vitro-induced germ cells) used for the experiments
- Washing and dilution of spermatocytes in PBS/1% BSA prior to Cytospin
- Cytospin parameters (112g for 7 minutes)
- Air drying and fixation of slides with 4% PFA
- Positive control: Omission of primary antibodies and replacement with mouse, rabbit and sheep isotype IgGs
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