Detailed Retinal Morphology Analysis

The protocol for mouse eye sections has been described previously^{66 (link)}. The eyes, including the appendages, were collected and fixed overnight in Davidson’s fixative (BBC Biochemical, Mount Vernon, WA, USA). The fixed eyes were excised to balance the pressure and preserve the morphology. They were then processed routinely and embedded in paraffin wax (ASP300S, Leica, Wetzlar, Germany). Subsequently, the eyes were cut into 7-μm retinal cross sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus, Tokyo, Japan). The retinal thickness was measured using the Nuance 3.02 software (PerkinElmer, Waltham, MA, USA).