Total RNA was isolated using the NucleoZOL RNA Isolation Reagent (Macherey–Nagel, Düren, Germany) and quantified with BioSpec-nano spectrophotometer (Shimadzu Inc.). Subsequently, cDNA synthesis and qRT-PCR were performed using the FastGene Scriptase II cDNA Synthesis 5 × Ready-Mix (NIPPON Genetics EUROPE, GmbH, Düren, Germany) and the KAPA SYBR FAST qPCR Master Mix (2X), respectively. Primers were designed using the primer-BLAST tool (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). Further details for the q-RT-PCR analysis are provided in [21 (link)].
Pateras I.S., Williams C., Gianniou D.D., Margetis A.T., Avgeris M., Rousakis P., Legaki A.I., Mirtschink P., Zhang W., Panoutsopoulou K., Delis A.D., Pagakis S.N., Tang W., Ambs S., Warpman Berglund U., Helleday T., Varvarigou A., Chatzigeorgiou A., Nordström A., Tsitsilonis O.E., Trougakos I.P., Gilthorpe J.D, & Frisan T. (2023). Short term starvation potentiates the efficacy of chemotherapy in triple negative breast cancer via metabolic reprogramming. Journal of Translational Medicine, 21, 169.
Other organizations :
Athens Naval & Veterans Hospital, TU Dresden, Umeå Plant Science Centre, Academy of Athens, Center for Cancer Research, Science for Life Laboratory, Karolinska Institutet, General University Hospital of Patras, University of Patras
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