Quantifying Soluble and Insoluble Dietary Fibers

The soluble and insoluble NSPs or xylan contents were measured as newly illustrated with minor modifications [18 (link)]. Ileal chyme samples were pretreated with fat extraction and enzymatic hydrolysis of starch. Subsequently, the supernatant and residue were subjected to different complicated steps such as hydrolysis, washing, centrifugation, and drying. The glycan degradation products were then analyzed for individual sugar concentrations by high-performance liquid chromatography (UPLC, Agilent 1200 series, Agilent Technologies, Santa Clara, CA, USA); the quantity of arabinose and xylose determined the AX content, and the total sugars represented the total NSP content. Monosaccharide standards consist of galactose (Gal), glucose (Glu), mannose (Man), arabinose (Ara), xylose (Xyl), fucose (Fuc), rhamnose (Rha), galacturonic acid (Glc), and glucuronic acid (GlcA) (Sigma-Aldrich Chemical Co., St. Louis, MO, USA), which were subjected to the same procedures as the samples.

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Wu W., Zhou H., Chen Y., Guo Y, & Yuan J. (2023). Debranching enzymes decomposed corn arabinoxylan into xylooligosaccharides and achieved prebiotic regulation of gut microbiota in broiler chickens. Journal of Animal Science and Biotechnology, 14, 34.

Publication 2023

Agilent 1200 series rhamnose glucuronic acid (GlcA)

Arabinose Centrifugation Enzymatic Fucose Galactose Galacturonic acid Glucose Glucuronic acid Glycan High performance liquid chromatography Hydrolysis Ileal Mannose Monosaccharide Rhamnose Starch Sugars Xylan Xylose

Corresponding Organization :

Other organizations : China Agricultural University

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Variable analysis

independent variables

Extraction and enzymatic hydrolysis of samples

dependent variables

Soluble and insoluble NSPs or xylan contents
Individual sugar concentrations (arabinose, xylose, galactose, glucose, mannose, arabinose, xylose, fucose, rhamnose, galacturonic acid, glucuronic acid)

control variables

Monosaccharide standards (galactose, glucose, mannose, arabinose, xylose, fucose, rhamnose, galacturonic acid, glucuronic acid)

controls

Positive control: Monosaccharide standards subjected to the same procedures as the samples
Negative control: Not explicitly mentioned

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