Splenocyte Activation by SIINFEKL and TGF-β

Splenocytes were cultured in vitro for 24 hours in 1nm SIINFEKL peptide or with 10ng/mL of TGF-β in RPMI containing 10% FBS.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Heim T.A., Lin Z., Steele M.M., Mudianto T, & Lund A.W. (2023). CXCR6 promotes dermal CD8+ T cell survival and transition to long-term tissue residence. bioRxiv.

Publication Preprint 2023

Siinfekl peptide Tgf β

Corresponding Organization : Oregon Health & Science University

Top 5 similar protocols

Variable analysis

independent variables

1nm SIINFEKL peptide
10ng/mL of TGF-β

dependent variables

Not explicitly mentioned

control variables

RPMI containing 10% FBS

controls

Positive control: not mentioned
Negative control: not mentioned

Annotations

This protocol is too short. Unable to provide accurate annotations

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!