RNA Extraction and Reverse Transcription

Total cell RNA was extracted by using Trizol reagent (Invitrogen, CA, USA). The reverse transcription and PCR process was performed according to the manufacturer’s instructions (Applied Biosystem, ThermoFisher Scientific, Shanghai, China). The whole procedure of the experiment was carried out according to our previous study [38 (link)]. The mRNA primers were designed and synthesized by the Sangon Biotech Co. Ltd. (Shanghai, China). And the miRNA primers were synthesized by RiboBio Co. Ltd (Guangzhou, China). The primer sequences were supplemented in Additional file 1: Table S4.

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Qiu B.Q., Lin X.H., Lai S.Q., Lu F., Lin K., Long X., Zhu S.Q., Zou H.X., Xu J.J., Liu J.C, & Wu Y.B. (2021). ITGB1-DT/ARNTL2 axis may be a novel biomarker in lung adenocarcinoma: a bioinformatics analysis and experimental validation. Cancer Cell International, 21, 665.

Publication 2021

Trizol reagent mRNA primers miRNA primers

Cell Mirna Mrna Primer Reverse transcription Trizol

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Nanchang University, Nanchang University, Ministry of Education of the People's Republic of China, First Affiliated Hospital of Nanchang University

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Variable analysis

independent variables

Total cell RNA extraction using Trizol reagent
Reverse transcription and PCR process according to the manufacturer's instructions

dependent variables

MRNA expression levels
MiRNA expression levels

control variables

Experimental procedure carried out according to previous study [38]
MRNA primers designed and synthesized by Sangon Biotech Co. Ltd.
MiRNA primers synthesized by RiboBio Co. Ltd.

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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