DNA Extraction from Plant Flour

DNeasy plant mini kit (Qiagen, Hilden, Germany) was chosen as a suitable procedure for DNA extraction based on the results of our previous study [33 (link)]. Genomic DNAs were isolated and purified from 100 mg flour. The purity and concentration of the extracted DNAs were estimated by spectrophotometer DeNovix DS-11 (DeNovix Inc. Wilmington, NC, USA). The quantity and integrity of DNA were evaluated using electrophoresis (VWR International, Radnor, PA, USA) on 1% agarose gel (SeaKem LE agarose; Cambrex, East Rutherford, NJ, USA) containing 1 μg/mL of ethidium bromide (Sigma-Aldrich, St. Louis, MI, USA). The agarose gels were visualized under ultraviolet (UV) light and a digital image was obtained using a gel documentation system PhotoDoc- It imaging system (UVP, Upland, CA, USA).