All procedures were approved by the Animal Care and Use Committee of Washington University and in strict accordance with the US National Institute of Health (NIH) Guide for the Care and Use of Laboratory Animals. Adult male and female mice (7-14 weeks of age) utilized in experiments were housed in Washington University School of Medicine animal facilities on a 12-hour light/dark cycle with access ad libitum to food and water.
For relevant optogenetic experiments, mice were generated with conditional expression of ChR2 in TrpV1 expressing sensory neurons by crossing heterozygous TrpV1-Cre mice (provided by Dr. Mark Hoon, NIDCR39 (link)) with homozygous Ai32 mice (Stock 3: 012569, The Jackson Laboratory)40 (link). For the purposes of this study, we refer to these mice as “TRPV1:ChR2”. These mice were previously characterized in our lab41 (link). All other experiments were performed using C57BL/6J mice bred in house, originally obtained from The Jackson Laboratory.
For relevant optogenetic experiments, mice were generated with conditional expression of ChR2 in TrpV1 expressing sensory neurons by crossing heterozygous TrpV1-Cre mice (provided by Dr. Mark Hoon, NIDCR39 (link)) with homozygous Ai32 mice (Stock 3: 012569, The Jackson Laboratory)40 (link). For the purposes of this study, we refer to these mice as “TRPV1:ChR2”. These mice were previously characterized in our lab41 (link). All other experiments were performed using C57BL/6J mice bred in house, originally obtained from The Jackson Laboratory.
