QDs (GSH–QDs) and annexinV–EGFP were prepared according
to the previously reported methods (
(Herceptin) and Kadcyla (trastuzumab emtansine) were purchased from
Chugai Pharmaceutical Co. Ltd. (Japan). The FITC-annexin V apoptosis
detection kit and MTT cell counting kit were purchased from Nacalai
Tesque (Japan). Fluorescent beads (size: 14 nm in diameter, latex,
FluoSpheres, carboxylate-modified and red fluorescent) were purchased
from Molecular Probes, Inc. Soybean lecithin was purchased from Nacalai
Tesque.
was purchased from Sigma. All other regents were of analytical grade
and were used as received without further purification. Breast tumor
cells (KPL-4) were kindly provided by Dr. J. Kurebayashi (Kawasaki
Medical School). Nude mice (5 week old female BALB/c nu/nu) were purchased
from Nihon SLC Inc. (Japan).