Glutathione-coated CdSeTe/CdS
QDs (GSH–QDs) and annexinV–EGFP were prepared according
to the previously reported methods (Supporting Information).64 (link),65 (link) The anti-HER2 monoclonal antibody
(Herceptin) and Kadcyla (trastuzumab emtansine) were purchased from
Chugai Pharmaceutical Co. Ltd. (Japan). The FITC-annexin V apoptosis
detection kit and MTT cell counting kit were purchased from Nacalai
Tesque (Japan). Fluorescent beads (size: 14 nm in diameter, latex,
FluoSpheres, carboxylate-modified and red fluorescent) were purchased
from Molecular Probes, Inc. Soybean lecithin was purchased from Nacalai
Tesque. l-α-Phosphatidyl-l-serine (soybean)
was purchased from Sigma. All other regents were of analytical grade
and were used as received without further purification. Breast tumor
cells (KPL-4) were kindly provided by Dr. J. Kurebayashi (Kawasaki
Medical School). Nude mice (5 week old female BALB/c nu/nu) were purchased
from Nihon SLC Inc. (Japan).