Longan Fruit Pericarp ADP, AMP, and ATP Analysis

Based on the protocols reported by Chen et al. (2018) and Zhang et al. (2019), 5 g of pericarp from 10 longan fruits were used to measure the contents of ADP, AMP, and ATP, and energy charge [30 (link),31 (link)], which was conducted by a high-performance liquid chromatography (LC-2030C, Shimadzu Corporation, Kyoto, Japan). The quantification of ADP, AMP, and ATP was expressed as mg/kg. Energy charge was computed using a formula: (ATP + 1/2 ADP)/(ATP + ADP + AMP).

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Sun J., Chen H., Chen Y., Lin M., Hung Y.C., Jiang Y, & Lin H. (2022). ε-Poly-l-Lysine Enhances Fruit Disease Resistance in Postharvest Longans (Dimocarpus longan Lour.) by Modulating Energy Status and ATPase Activity. Foods, 11(5), 773.

Publication 2022

LC-2030C

Fruits High performance liquid chromatography Longan

Corresponding Organization : Fujian Agriculture and Forestry University

Other organizations : Quanzhou Normal University, University of Missouri, University of Georgia

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Variable analysis

independent variables

Amount of pericarp used (5 g)

dependent variables

Contents of ADP
Contents of AMP
Contents of ATP
Energy charge

control variables

Number of longan fruits used (10)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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