Quantification of lncRNA RMRP Expression

Total RNA of tissues or cells was extracted using Trizol Reagent (Life Technologies). RNA was reversed transcribed into cDNAs using the Primer-Script one step RT-PCR kit (TaKaRa). The cDNA template was amplified by real-time RT-PCR using the SYBR Premix Dimmer Eraser kit (TaKaRa). GAPDH was used as an internal control, and RMRP values were normalized to GAPDH. qRT-PCR reactions were performed by the ABI7500 system (Applied Biosystems). The relative expression fold change of mRNAs was calculated by the 2^–ΔΔCt method. The primer sequences were as follows: lncRNA RMRP: 5′-ACTCCAAAGTCCGCCAAGA-3′ and 5′-GTAACTAGAGGGAGCTGAC-3′; GADPH: 5′-GTCAA-CGGATTTGGTCTGTATT-3′ and 5′-AGTCTTCTGGGTGG-CAGTGAT-3′ [11 (link)].