Both mouse microglia (BV2 cells) and neuron (N2a cells) were purchased from Cell
Center, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing, China. BV2
cells were cultured in DMEM medium (Gibco), which was supplemented with 10%
fetal bovine serum (Gibco), and 1% penicillin-streptomycin (Gibco). The cells
were then incubated at 37°C in a humidified atmosphere of 5% CO2. For
M1 microglia, microglial cells were stimulated with 100 ng/mL LPS + 20 ng/mL
IFN-γ (PeproTech),19 (link),20 (link) and then treated with vehicle or candesartan (Sigma). For
M2 microglia, microglial cells were induced with 20 ng/mL IL-4 (PeproTech).20 (link) After 24 h treatment, cells were harvested for testing the changes of
mRNA and protein level, and the supernatant was collected for measuring the
level of inflammatory cytokines.
Center, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing, China. BV2
cells were cultured in DMEM medium (Gibco), which was supplemented with 10%
fetal bovine serum (Gibco), and 1% penicillin-streptomycin (Gibco). The cells
were then incubated at 37°C in a humidified atmosphere of 5% CO2. For
M1 microglia, microglial cells were stimulated with 100 ng/mL LPS + 20 ng/mL
IFN-γ (PeproTech),19 (link),20 (link) and then treated with vehicle or candesartan (Sigma). For
M2 microglia, microglial cells were induced with 20 ng/mL IL-4 (PeproTech).20 (link) After 24 h treatment, cells were harvested for testing the changes of
mRNA and protein level, and the supernatant was collected for measuring the
level of inflammatory cytokines.
