The neural-tube slices were incubated with an anti-phosphohistone H3 (H3P)
antibody (Upstate, Charlottesville, VA; 1:100 dilution), followed by a
fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG antibody (Jackson
Immunoresearch, West Grove, PA). All the antibodies were incubated with the
slices for 1 h at room temperature in 1% goat serum in PBST (PBS with 10% Tween
20), and the secondary antibody was diluted 1 to 200 (23) (link). The cells were counterstained with
4',6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA) to
visualize the nuclei.
antibody (Upstate, Charlottesville, VA; 1:100 dilution), followed by a
fluorescein isothiocyanate (FITC)-conjugated anti-rabbit IgG antibody (Jackson
Immunoresearch, West Grove, PA). All the antibodies were incubated with the
slices for 1 h at room temperature in 1% goat serum in PBST (PBS with 10% Tween
20), and the secondary antibody was diluted 1 to 200 (23) (link). The cells were counterstained with
4',6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA) to
visualize the nuclei.
