Human diffuse large B-cell lymphoma OCI-Ly8 and B-ALL Reh cell lines were cultured and maintained in RPMI1640 medium supplemented with 10% FBS, 2 mmol/L l -glutamine, and antibiotic–antimycotic at 37°C and 5% CO2. The use of Reh cells has been described previously (29 (link)). The OCI-Ly8 cell line was a kind gift from the laboratory of Raju Chaganti (Memorial Sloan Kettering Cancer Center, New York, NY). Cell line authentication for OCI-Ly8 and Reh was performed by short tandem repeat profiling in 2021. All cell lines were routinely confirmed to be Mycoplasma free via Sartorius EZ-PCR Mycoplasma Detection Kit.
Protocol detail
Cell Culture of Lymphoma and Leukemia Lines
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Antibiotic
B all
Cancer
Cell line authentication
Cell lines
Diffuse large b cell lymphoma
Glutamine
Human
Mycoplasma
Short tandem repeat
Corresponding Organization : University of Pennsylvania
Other organizations : Cincinnati Children's Hospital Medical Center, University of Cincinnati
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Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Cell culture medium (RPMI1640)
- Fetal bovine serum (10%)
- L-glutamine (2 mmol/L)
- Antibiotic–antimycotic
- Temperature (37°C)
- CO2 concentration (5%)
- None explicitly mentioned
- None explicitly mentioned
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