Western Blot Analysis of Cell Cycle Regulators

Antibodies used included mouse anti-Wee1, mouse anti-P53 (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.), rabbit anti-phospho-CDK1 (Y15), rabbit anti-phospho-H2AX (S139), mouse anti-phospho-Histone H3 (S10), rabbit anti-Histone H3 (Cell Signaling Technology, Danvers, MA, U.S.A.), mouse anti-CDK1 (Millipore, Billerica, MA, U.S.A.), mouse anti-P21 (BD Biosciences, San Diego, CA, U.S.A.), and mouse anti-Actin (Sigma, St. Louis, MO, U.S.A.). Western blots were performed as previously described [13 (link)].

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Pappano W.N., Zhang Q., Tucker L.A., Tse C, & Wang J. (2014). Genetic inhibition of the atypical kinase Wee1 selectively drives apoptosis of p53 inactive tumor cells. BMC Cancer, 14, 430.

Publication 2014

mouse anti-P53 rabbit anti-phospho-H2AX mouse anti-phospho-Histone H3 rabbit anti-Histone H3 mouse anti-P21 mouse anti-Actin

Actin Antibodies Cdk1 Histone h3 Mouse Rabbit Western blots

Corresponding Organization :

Other organizations : AbbVie (United States)

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Variable analysis

independent variables

Antibodies used: mouse anti-Wee1, mouse anti-P53 (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.), rabbit anti-phospho-CDK1 (Y15), rabbit anti-phospho-H2AX (S139), mouse anti-phospho-Histone H3 (S10), rabbit anti-Histone H3 (Cell Signaling Technology, Danvers, MA, U.S.A.), mouse anti-CDK1 (Millipore, Billerica, MA, U.S.A.), mouse anti-P21 (BD Biosciences, San Diego, CA, U.S.A.), and mouse anti-Actin (Sigma, St. Louis, MO, U.S.A.)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

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