Immunostaining of Developing Teeth

Offspring were collected at E15.5, E17.5, and P0.5. Paraffin sections were prepared and immunostaining was performed as previously described23 (link). Primary antibodies against Ki67 (ab15580, Abcam), AMBN (sc-50534, Santa Cruz), AMGN (sc-32892, Santa Cruz), and APEX1 (ab194, Abcam) were used. To determine the cell proliferation index, Ki67-positive cells in the cervical loop of each tissue section were counted. At least five serial sections from each animal were examined, and three animals from different litters (n = 3) were included in each group.

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Chen G., Chen J., Yan Z., Li Z., Yu M., Guo W, & Tian W. (2017). Maternal diabetes modulates dental epithelial stem cells proliferation and self-renewal in offspring through apurinic/apyrimidinicendonuclease 1-mediated DNA methylation. Scientific Reports, 7, 40762.

Publication 2017

ab15580 sc-50534 sc-32892 ab194

Animals Antibodies Apex1 Cell proliferation Cells Cervical Litters Paraffin Tissue

Corresponding Organization :

Other organizations : Sichuan University

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Variable analysis

independent variables

Developmental stage (E15.5, E17.5, P0.5)

dependent variables

Cell proliferation index (percentage of Ki67-positive cells in the cervical loop)

control variables

Tissue sections (at least five serial sections from each animal)
Number of animals (three animals from different litters)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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