Iron-Mediated Oxidative Stress Pathways

Reagents included ammonium iron(III) citrate (Sigma Aldrich, F5879), ferric chloride (Sigma Aldrich, 44939), 2,2-bipyridyl (Sigma Aldrich, 366-18-7), 4,4-bipyridyl (Sigma Aldrich, 533-26-4), hydrogen peroxide (Sigma Aldrich, H10009), CID1067700 (Sigma Aldrich, 1067700), dynasore hydrate (Sigma Aldrich, D7693), bafilomycin A₁ (Sigma Aldrich, B1793), nigericin (Sigma Aldrich, N7143), iron dextran (Sigma Aldrich, D8517), BAPTA-AM (Invitrogen, B6769), acridine orange (Calbiochem, 113000), and torin 1 (Tocris Bioscience, 4247). Trans-Ned-19 and a cell-permeant version of NAADP (NAADP-AM) were synthesized as previously described.^28,76 (link)

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Fernández B., Fdez E., Gómez-Suaga P., Gil F., Molina-Villalba I., Ferrer I., Patel S., Churchill G.C, & Hilfiker S. (2016). Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy, 12(9), 1487-1506.

Publication 2016

ammonium iron(III) citrate ferric chloride 2,2-bipyridyl hydrogen peroxide CID1067700 dynasore hydrate bafilomycin A1 nigericin iron dextran BAPTA-AM torin 1

Acridine orange Ammonium Bafilomycin a1 Bapta Bipyridyl Cell Cid1067700 Citrate Dynasore Ferric chloride Hydrogen peroxide Iron Iron dextran Naadp Ned 19 Nigericin Torin

Corresponding Organization : Consejo Superior de Investigaciones Científicas

Other organizations : Universidad de Granada, Bellvitge University Hospital, Universitat de Barcelona, Institut d'Investigació Biomédica de Bellvitge, University College London, University of Oxford

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Variable analysis

independent variables

Ammonium iron(III) citrate
Ferric chloride
2,2-bipyridyl
4,4-bipyridyl
Hydrogen peroxide
CID1067700
Dynasore hydrate
Bafilomycin A1
Nigericin
Iron dextran
BAPTA-AM
Acridine orange
Torin 1
Trans-Ned-19
NAADP-AM

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

Annotations

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