Quantitative Proteomics Analysis via DIGE Imaging

Gels were scanned with the Ettan DIGE Imager (software 1.0; GE Healthcare) and generated gel image triplets (Cy2, Cy3, and Cy5) comprising the CyDye-labeled proteins. Quantitative analysis was carried out with the DeCyder 2D difference analysis software (Version 7.0; GE Healthcare). Spot detection and matching was performed automatically with the DeCyder Batch processor. The gel-to-gel matching was also checked manually followed by statistical analysis of protein abundance change between samples in the biological variation analysis (BVA) module embedded in the DeCyder Software [37 (link), 79 (link), 80 ]. Spots of interest differentially expressed at least in two comparisons with p < 0.05, and spots differentially expressed in one comparison with p < 0.01 were further analyzed with MS (39 spots fulfilled the criteria, Fig. 1b).

Free full text: Click here

Laskowska-Macios K., Nys J., Hu T.T., Zapasnik M., Van der Perren A., Kossut M., Burnat K, & Arckens L. (2015). Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat. Molecular Brain, 8, 48.

Publication 2015

Ettan DIGE Imager DeCyder 2D difference analysis software

Biological Protein change Proteins Spots Triplets

Corresponding Organization :

Other organizations : Instytut Biologii Doświadczalnej im. Marcelego Nenckiego, KU Leuven

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein abundance change between samples

control variables

None explicitly mentioned

controls

No positive or negative controls were mentioned in the given information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!