Tracking Metastatic Potential of DAB2IP-Knockdown PC3 Cells

Early study has shown that DAB2IP gene knockdown in PC3 cell, a poorly-metastatic line, increases its metastatic potential (also called as DAB2IP-knockdown PC3 cells or KD cells)²⁴ . These stable cell lines expressing dual reporter genes (GFP and luciferase) were maintained in RPMI1640 medium (Invitrogen, Carlsbad, CA, USA) containing 5% PBS as previous described²⁴ . Migration assays were performed in Transwell dishes (Corning Costar, Cambridge, MA, USA) as described earlier^{73 (link)}. For tracking the cell migration in vivo, both PC3 and KD were labeled with Qtracker® labeling kit (Life technologies, Carlsbad, CA, USA) by following manufacture's instruction as illustrated earlier^{74 (link)}. For histological study, Vybrant DiD cell labeling dye (Thermo Fisher Scientific, Waltham, MA, USA) was used to track cancer cells according to the product information for observation under microscope^{75 (link)}.