Flow cytometric analysis was performed using a FACSCanto II flow cytometer and Kaluza 1.5 software (Beckman Coulter, Brea, CA, USA); 10,000 cells of each sample were acquired. Prior to multicolor staining, the compensation was set using single-positive cells for each color.
Flow Cytometric Immune Cell Analysis
Flow cytometric analysis was performed using a FACSCanto II flow cytometer and Kaluza 1.5 software (Beckman Coulter, Brea, CA, USA); 10,000 cells of each sample were acquired. Prior to multicolor staining, the compensation was set using single-positive cells for each color.
Corresponding Organization : Warsaw University of Life Sciences
Variable analysis
- Gating strategy for doublets removal
- Gating strategy for lymphocytes vs. monocytes
- Gating strategy for CD4+, CD8+, and FoxP3+ cells
- Gating strategy for CD5+, CD14+, MHCII+ cells
- Cell proliferation
- Median fluorescence intensity (MFI) of ROS
- Flow cytometric analysis using a FACSCanto II flow cytometer
- Kaluza 1.5 software (Beckman Coulter, Brea, CA, USA)
- Acquisition of 10,000 cells per sample
- Compensation set using single-positive cells for each color
- Positive controls: Single-positive cells for each color
- Negative controls: Not explicitly mentioned
Annotations
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