TEM Sample Preparation Protocol

A TEM sample was prepared with minor modifications from the previous study (Golding et al., 2016 (link)). The sample was adsorbed for 1 min to a formvar film on a carbon-coated 400-mesh copper grid. It was then washed 3 times in distilled water and negatively contrasted with 2% methylamine tungstate (Nano-W; Nanoprobes, Yaphank, NY, United States). The image was taken at 200 kV using a FEI Tecnai 20 transmission electron microscope (FEI Company, Hillsboro, OR, United States). The magnifications of 3,500× to 19,000× was considered for TEM images.

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Ashrafudoulla M., Mizan M.F., Park H., Byun K.H., Lee N., Park S.H, & Ha S.D. (2019). Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel. Frontiers in Microbiology, 10, 513.

Publication 2019

Nano-W FEI Tecnai 20

Carbon Copper Formvar Methylamine tungstate Transmission electron microscope

Corresponding Organization :

Other organizations : Chung-Ang University, Korea Food Research Institute, Oregon State University

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Variable analysis

independent variables

Adsorption time (1 min)

dependent variables

Morphology of the sample (observed through TEM images)

control variables

Formvar film on a carbon-coated 400-mesh copper grid
Washing the sample 3 times in distilled water
Negative staining with 2% methylamine tungstate
Imaging at 200 kV using a FEI Tecnai 20 transmission electron microscope
Magnification range of 3,500× to 19,000×

controls

No positive or negative controls were explicitly mentioned in the provided information.

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