Immunofluorescence Staining of Neural Markers

For immunofluorescence, cells were fixed with 4% paraformaldehyde and permeabilized in 0.3% Triton X-100. Antibodies included rabbit anti-TLX (1:1,000; Shi lab)45 (link), mouse anti-nestin (1:2,000; BD Pharmingen; Catalogue # 611659)55 (link), rabbit anti-integrin αv (1:500; Chemicon; Catalogue # AB1923)43 (link), mouse anti-neuropilin-1 (1:11; Miltenyi Biotec; Catalogue # 130-090-693)43 (link), mouse anti-GFAP (1:1,000; Sigma; Catalogue # G3893) and rabbit anti-Tuj1 (1:6,000; Covance; Catalogue # PRB-435P)56 (link).

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Cui Q., Yang S., Ye P., Tian E., Sun G., Zhou J., Sun G., Liu X., Chen C., Murai K., Zhao C., Azizian K.T., Yang L., Warden C., Wu X., D'Apuzzo M., Brown C., Badie B., Peng L., Riggs A.D., Rossi J.J, & Shi Y. (2016). Downregulation of TLX induces TET3 expression and inhibits glioblastoma stem cell self-renewal and tumorigenesis. Nature Communications, 7, 10637.

Publication 2016

mouse anti-nestin mouse anti-GFAP rabbit anti-Tuj1

Antibodies Cells Gfap Immunofluorescence Integrin αv Mouse Nestin Neuropilin 1 Paraformaldehyde Rabbit Triton x 100

Corresponding Organization :

Other organizations : City of Hope, Centre Interdisciplinaire de Nanoscience de Marseille

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Variable analysis

independent variables

Antibodies used for immunofluorescence staining

dependent variables

Protein expression and localization of TLX, nestin, integrin αv, neuropilin-1, GFAP, and Tuj1

control variables

Cells were fixed with 4% paraformaldehyde
Cells were permeabilized in 0.3% Triton X-100

controls

No positive or negative controls were explicitly mentioned in the provided information.

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